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Chettri Prabha, Wangchu L., Nimbolkar P. K., Singh Siddhartha, Rozerto Khwairakpam, Dhanalakshmi S.
Keywords: n Hylocereus costaricensisn , IBA, Stem cuttings, Vegetative propagation, Survival percentage
This research sought to establish standardized protocols for the cutting types, planting time and effects of IBA application on improving the propagation of dragon fruit (Hylocereus costaricensis). Shoot cuttings with slanting cuts, exposing the interior wood portion, were made and planted in May, June, and July after treating with IBA concentrations of 5000 ppm, 6000 ppm, 7000 ppm and 8000 ppm. Observations of shoot and root growth were recorded ninety days after planting of cuttings. The interaction of IBA, type of cut, and planting months showed the highest survival percentage (97.80%), percentage of rooting (98.9%), root length (29.60 cm), shoot length (42.83 cm), number of shoots (2.33), average length of shoots per cutting (42.83 cm), shoot diameter (29.30 mm), and fresh weight of shoots (105.93 g) with 7000 ppm IBA treated on a shoot with slanting cut and planted during the month of June. The IBA at 7000 ppm applied to shoot cuttings exposing the interior wood portion and planted during month of June showed better results in terms of carbohydrates (0.35%), lipids (118.03 mg/100 g), and total chlorophyll (142.9 mg/100 g). Regarding rooting performance, the number of primary roots per cutting (11.30), as well as the fresh (1.78 g) and dry (0.47 g) weight of roots, were better with IBA at 5000 ppm and cuttings exposing the interior wood portion which was planted during the month of June, while the longest root length (29.60 cm) was observed with IBA at 7000 ppm applied to the shoots with slanting cuts and planted during the month of May. The overall study concluded that the application of IBA at 7000 ppm on a slanting cut space of the shoots is effective when planted during the month of June for the propagation of dragon fruit.
Ali SI, Kumar TS, Kumar AK, Joshi V, Kumar BN (2022) Studies on effect of different concentrations of IBA and length of cuttings on rooting and shoot growth performance in Dragon fruit Hylocereus spp.-red flesh with Pink skin under Telangana conditions. J Pharm Innov 11(3):738–743
Baghel M, Raut U, Ramteke V (2016) Effect of IBA concentrations and time of air-layering in guava cv. L-49. Res J Agri Sci 7(1):117–120
Babaie H, Zarei H, Nikdel K, Firoozjai MN (2014) Effect of different concentrations of IBA and time of taking cutting on rooting, growth and survival of Ficus Binnendijkii ‘Amstel queen’ cuttings. Not Sci Biol 6(2):163–166
Bennett T, Hines G, Leyser O (2014) Canalization: what the flux? Trends Genet 30:41–48. https://doi.org/10.1016/j.tig.2013.11.001
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917
Bielach A, Hrtyan M, Tognetti VB (2017) Plants under stress: involvement of auxin and cytokinin. Int J Mol Sci 18(7):1427. https://doi.org/10.3390/ijms18071427
Das Shubranil S, Shil J, Rime AK, Alice T, Yumkhaibam V, Mounika AP, Singh et al (2025) Phytohormonal signaling in plant resilience: advances and strategies for enhancing abiotic stress tolerance. J Plant Growth Regul 105:329–360. https://doi.org/10.1007/s10725-025-01279-6
De Klerk GJ, Van Der Krieken W, De Jong JC (1999) Review the formation of adventitious roots: new concepts, new possibilities. Vitro Cell Dev Biol Plant 35:189–199
Demmig-Adams B, Adams III, WW (2002) Antioxidants in photosynthesis and human nutrition. Science 298(5601):2149–2153. https://doi.org/10.1126/science.1078002
Dhruve L, Suchitra V, Vani VS, Subbaramamma P, Saravanan L (2018) Rooting and shooting behavior of red and white pulped varieties of Dragon fruit (Hylocereus undatus) in relation to Indole 3- Butyric acid concentrations. Int J Agric Sci 14:229–234. https://doi.org/10.15740/HAS/IJAS/14.1/229-234
Esquivel P, Stintzing FC, Carle R (2007) Comparison of morphological and chemical fruit traits from different Pitaya genotypes (Hylocereus sp.) grown in Costa Rica. J Appl Bot Food Qual 81:7–14. https://doi.org/10.5073/JABFQ.2007.081.002
Frick EM, Strader LC (2018) Roles for IBA-derived auxin in plant development. J Exp Bot 69(2):169–177. https://doi.org/10.1093/jxb/erx298
Gomez AK, Gomez AA (2010) Statistical procedures for agricultural research, 2nd edn. Wiley India Pvt. Ltd., New Delhi, pp 134–138
Hedge JE, Hofreiter BT (1962) In: Whistler RL, Miller B (eds) Methods in carbohydrate chemistry, vol 17. (Eds.,). Academic, New York, pp. 420
Hartmann HT, Kester DE, Davies FT, Geneve RL (1997) In Book: Plant propagation: principles and practices 6: 770–772
Karunakaran G, Arivalagan M, Sriram S (2019) Dragon fruit country report from India. ICAR- Indian Institute of Horticultural Research, Bengaluru, Karnataka, India
Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoid: measurement and characterization by UV-spectroscopy. CPFA, F4.3.1-F4.3.8
Lichtenthaler HK, Lang M, Sowinska M, Heisel F, Miehe JA (1996) Detection of vegetation stress via a new high resolution fluorescence imaging system. J Plant Physiol 148(5):599–612. https://doi.org/10.1016/S0176-1617(96)80081-2
Mai Thi M, Ha DH, Nguyen N, Chieu (2025) Expanding export markets for Vietnamese Dragon fruit. IJSRM 13:8344–8352. https://doi.org/10.18535/ijsrm/v13i02.em03
Mansouri H, Talebizadeh R (2017) Effects of indole-3‐butyric acid on growth, pigments and UV‐screening compounds in Nostoc Linckia. Phycol Res 65(3):212–216. https://doi.org/10.1111/pre.12177
Minz V, Panigrahi HK, Sangeeta (2021) Effect of different plant growth regulators and media on shooting of stem cuttings in Dragon fruit. J Pharm Innov 10(8):330–332
Morton J (1987) Cactaceae: strawberry pear and related species. In: Fruits of Warm Climates 347 – 48
Ou Yang F, Wang J, Li Y (2015) Effects of cutting size and exogenous hormone treatment on rooting of shoot cuttings in Norway Spruce [Picea abies (L.) Karst]. New Forest 46:91–105. https://doi.org/10.1007/s11056-014-9449-1
Pushpakumara DKNG, Gunasena HPM, Kariayawasam R (2009) Proximate analysis of Dragon fruit (Hylocereus polyrhizus). Amer. J App Sci 7:1341–1346. https://doi.org/10.3844/ajassp.2009.1341.1346
Rahad MK, Islam MA, Rahim MA, Monira S (2016) Effects of rooting media and varieties on rooting performance of Dragon fruit cuttings (Hylocereus Undatus Haw). Res Agric Livest Fish 3(1):67–77. https://doi.org/10.3329/ralf.v3i1.27859
Raspor M, Motyka V, Kaleri AR, Ninkovic S, Tubic L, Cingel A, Cosic T (2021) Integrating the roles for cytokinin and auxin in de Novo shoot organogenesis: from hormone uptake to signaling outputs. Int J Mol Sci 22(16):8554. https://doi.org/10.3390/ijms22168554
Raut UA, Rathod SR, Bharad SG, More PG (2022) Study of different IBA levels on nursery behaviour of Dragon fruit cutting. J Pharm Innov 11(8):346–351
Siddiqua A, Thippesha D, Reddy MV, Raj ND (2019) Effect of different plant growth regulators on shooting of stem cuttings in Dragon fruit [ Hylocereus Undatus (Haworth) Britton & Rose]. Int J Curr Microbiol App Sci 8(5):1621–1627. https://doi.org/10.20546/ijcmas.2019.805.187
Singh AK, Singh VS (2002) Influence of wood maturity and auxins on the regeneration of Bougainvillea cuttings. Prog Hort 34(2):196–199. https://doi.org/10.24154/jhs.v7i2.378
Tripathi PC, Karumakaran G, Sankar V, Senthikumar R (2014) Dragon Fruit: Nutritive and ruminative fruit. Bulletin, Central Hort. Exp. Station: IIHR, Chettalli- 571248, Kodagu, Karnataka: 1–11
Ullah T, Wazir FU, Ahamad M, Analoui F, Khan MU, Ahmad M (2005) A breakthrough in guava (Psidium Guajava L.) propagation from cutting. Asian J Pl Sci 4(3):238–243
Upadhayay PK, Kharal S, Shrestha B (2020) Effect of indole-butyric acid (IBA) and wounding on rooting ability and vegetative characteristics of apple rootstock cuttings under Nepal conditions. J Agric Sci 5(4): 184–192. https://doi.org/10.31248/JASP2020.220
Wu S, Rongguang Li C, Bu C, Zhu C, Miao Y, Zhang J, Cui Y, Jiang X Ding (2024) Photoperiodic effect on Growth, Photosynthesis, mineral Elements, and metabolome of tomato seedlings in a plant factory. Plants 13(22):3119. https://doi.org/10.3390/plants13223119
Zhou J, Li P, Wang J (2022) Effects of light intensity and temperature on the photosynthesis characteristics and yield of lettuce. Horticulturae 8(2):178. https://doi.org/10.3390/horticulturae8020178
Department of Fruit Science, College of Horticulture & Forestry, Pasighat, India